Associate Professor Bernard Hughes

• Biochemistry, Biotechnology, Clinical Chemistry

Australian Society of Biochemistry and Molecular Biology

B.Sc Adelaide,1975; B.Sc (Hons) Adelaide,1976; PhD Flinders, 1980

• Structure and Function of Ion Channels: Molecular Basis of Myotonic Muscle Diseases: Engineering of Proteins and their Expression in Heterologous Systems

REPRESENTATIVE PUBLICATIONS 38. Hryciw,D.H., Rychkov,G.Y., Hughes,B.P. & Bretag,A.H., Relevance of the D13 region to the function of the skeletal muscle chloride channel, ClC-1, Journal of Biological Chemistry 273, 4304-4307 (1998)

37. Rychkov,G.Y., Astill,D.St.J., Bennetts,B., Hughes,B.P., Bretag,A.H. & Roberts,M.L., pH-dependent interactions of Cd2+ and a carboxylate blocker with the rat ClC-1 chloride channel and its R304E mutant in the Sf-9 insect cell line, Journal of Physiology 501.2, 355-362, (1997)

35. Astill,D.St.J., Rychkov,G., Clarke,J.D., Roberts,M.L., Hughes,B.P. & Bretag,A.H., Characteristics of skeletal muscle chloride channel ClC-1 and point mutation R304E expressed in Sf9 insect cells, Biochimica et Biophysica Acta, 1280, (2), 178-186, (1996)

34. Auld,A.M., Bawden,M.J., Berven,L.A., Harland,L., Hughes,B.P. & Barritt,G.J., Injection of rat hepatocyte poly(A)+ RNA to Xenopus laevis oocytes leads to expression of a constitutively-active divalent cation channel distinguishable from endogenous receptor-activated channels, Cell Calcium, 19, (5), 439-452, (1996)

33. Berven,L.A., Hughes,B.P. & Barritt,G.J., A slowly ADP-ribosylated pertussis-toxin-sensitive GTP regulatory protein is required for vasopressin- stimulated Ca2+ inflow in hepatocytes, Biochemical Journal, 299, 399-407, (1994)

31. Strazzari,M.J. & Hughes, B.P., Evidence that the Ca2+ inflow pathway in hepatocytes stimulated by thapsigargin is similar to that activated by vasopressin, Biochemical Pharmacology, 45, (10), 2163-2165, (1993)

39. Aromataris,E.C., Rychkov,G.Y., Bennetts,B., Hughes,B.P., Bretag,A.H. & Roberts,M.L., Fast and slow gating of ClC-1: Differential effects of 2 (4-chlorophenoxy) propionic acid and dominant negative mutations, Molecular Pharmacology 60, 200-208 (2001)

40. Simpson, B., Height, T., Rychkov, G. Y., Nowak, K., Laing, N., Hughes,B.P. & Bretag, A. H., Characterization of three myotonia-associated mutations of the CLCN1 chloride channel gene via heterologous expression, Human Mutation, Mutation in Brief #730, online (2004)

41. Wu,W.P., Rychkov, G.Y., Hughes, B.P. & Bretag, A.H., Functional complementation of truncated human ClC-1 using carboxyl tail fragments, Biochemical Journal 395 (1), 89-97 (2006).

42. Ma, L., Rychkov, G.Y., Hughes, B.P. & Bretag, A.H., Analysis of carboxyl tail function in the skeletal muscle channel, hClC-1 Biochemical Journal 413 (1), 61-69 (2008).

I am able to provide media comment in the following areas of expertise:

Discipline: Biochemistry

• Ion Channels
• Proteins
• Cell Signalling
• Genetic Engineering